Journal: Current Research in Food Science

Article Title: A Pectic Polysaccharide from Lycium ruthenicum Murray Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice

doi: 10.1016/j.crfs.2024.100955

Figure Lengend Snippet: Fig. 4. Effects of LRWP-Ap on pathological changes and TJ protein expression and on the balance of Arg-1/iNOS in mouse colon tissues. (A&B) H&E staining of mouse colon tissue. H&E staining (A) and colonic tissue damage score (B). (C–F) Western blot analysis of TJ protein and β-actin protein expression in colon tissues. Grayscale intensity statistics for Claudin 1 (D), Occludin (E), and ZO-1 (F) protein expression. (G–I) Immunofluorescence images of Arg-1, iNOS, and DAPI in paraffin- embedded colon tissue sections from each group. Relative fluorescence intensity of Arg-1 (H) and iNOS (I). L–A: LRWP-Ap. The data are expressed as the means ± standard deviations (n = 3, randomly selected from 6). *P < 0.05 and **P < 0.01 vs. the control group. #P < 0.05 and ##P < 0.01 vs. the model group.

Article Snippet: The membrane was blocked with a Rapid Closure Solution (GeneFist, Shanghai, China) for 30 min, then incubated overnight at 4◦Cwith primary antibodies against ZO-1, Occludin, Claudin-1, and β-actin (Proteintech, Hubei, China).

Techniques: Expressing, Staining, Western Blot, Immunofluorescence, Fluorescence, Control

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 1 The map of the recombinant vector for expression of His-CDH1 fusion peptide. The CDH1 ectodomain was link to the His-tag in the pET-44a(+) vector. Expression of His-CDH1 fusion peptide was driven by T7 lac promoter.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Recombinant, Plasmid Preparation, Expressing

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 2 Analysis of the optimal expressed condition of His-CDH1 fusion peptide by SDS-PAGE. M, protein molecular weight marker; 1, control; 2-9, the sample induced by IPTG at 1, 1.5, 2, 2.5, 3, 4, 5 and 6 h, respectively.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: SDS Page, Molecular Weight, Marker, Control

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 3 Western blot analysis of the condition for purifying His- CDH1 peptide using Ni-NTA chromatography. 1, prestained protein marker; 2, the recovery fluid with lysate supernatant; 3, the recovery fluid with 20 mmol imidazol; 4-5, the recovery fluid with 100 mmol imidazol; 6-7, the recovery fluid with 250 mmol imidazol; 8-9, the recovery fluid with 500 mmol imidazol; 10, the recovery fluid with 1 000 mmol imidazol.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Western Blot, Chromatography, Marker

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 4 Ability of the sheep CDH1 polyclonal antibodies was detected by Western blotting. Total protein was from sheep testis (T), kinedy (K), pulmo (P) and liver (L). There were three bands in 135, 120 and 80 kDa.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Western Blot

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 5 Distribution patterns of CDH1 and PLZF in cross sections of 5-mon-old sheep testis. A, immunohistochemistry examination of PLZF expressing cells within cross sections of seminiferous tubules from 5-mon-old sheep testis. B, immunohistochemistry examination of CDH1 in the same section. C, the nuclei of the cell sections were stained by Hoechst 33342. D, merged image of A, B and C. Some PLZF- and CDH1-positive cells comprising single (diamond arrows) and paired spermatogonia (arrows) were localized at the basement membrane of the seminiferous tubules. Scale bars are 40 μm.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Immunohistochemistry, Expressing, Staining, Membrane

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 6 Whole-mount immunohistochemistry of 5-mon-old sheep seminiferous tubules. The CDH1-positive cells were small in number, there are single cells attached the basement membrane of the seminiferous tubules (arrows), and there are paired cells attached each other (diamond arrow). Scale bar is 200 μm.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Immunohistochemistry, Membrane